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Jan 07, 2021 · Background The dentin is a tissue, which is formed by odontoblasts at the pulp interface of the teeth that supports the enamel. Odontoblasts, the cranial neural crest cells are derived from ectodermal mesenchymal stem cells (MSCs) and are long and polarized cells. They are present at the outer surface of dentin and play a prominent role about dentin formation. Recently, attention has been

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InductOS, Wyeth/Pfizer, New York,NY,USA). Inaddition,osteogenic differentiationmedium (ODM) wasused,consisting of10mMofβ-glycerophosphateand 10nMdexamethasone/0.2 mML-ascorbicacid 2-phosphate (Sigma-Aldrich).Medium waschangedevery3–4days. Alkalinephosphataseactivity ForALPstaining,cells werefixed in4%(w/v) paraformaldehydeand permeabilizedin

Aug 01, 2015 · Stem cells from the human dental apical papilla (SCAP) can be obtained from almost all extracted wisdom teeth with an immature tooth root. Although different stem cell lines are used for studies, it remains elusive whether specific characteristics of the dental stem cell cultures such as proliferation rates or the cell differentiation potential are related to the cell source, e.g. the donor

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Aug 15, 2006 · The lack of osteogenesis was further confirmed by gene analysis after 21 days in ODM, examining expression patterns for the osteogenic differentiation markers runx2/cbfa1 (Runx2), osteopontin (Opn), and osteocalcin (Ocn) (Fig. 7A, which is published as supporting information on the PNAS web site). No significant induction in any of these genes

Exposure to ODM results in morphological change and acqui-sition of neural progenitor phenotype. Undifferentiated hfMSC did not express specific early or late markers of oligodendrocyte differentiation, but did express low levels of nestin and vimentin. Following exposure to ODM there was a dramatic morphological

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Dec 13, 2018 · Insufficient bone volume remains a key issue when using dental implants. Adipose tissue‑derived stem cells (ADSCs) can accelerate bone healing when combined with dental implants. To improve the application of ADSCs for dental uses, the present study aimed to identify optimal implantation conditions. Mesenchymal stem cell‑derived exosomes can induce naïve stem cells to differentiate

Nov 25, 2014 · Cell culture. All cell culture media and supplements were obtained from Gibco (Carlsbad, CA, USA), unless otherwise indicated. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were purchased from ScienCell Research Laboratories (Cat. No. 7500; Carlsbad, CA, USA) and maintained in growth medium (GM) consisting of α-Minimum Essential Medium (α-MEM) supplemented with 16.5% fetal bovine

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As its name implies, this is a tapered thread so that the outside diameter of the male (ODM) gets smaller towards the end of the fitting and the inside diameter of the female narrows with depth. As the two fittings are threaded together the opposite tapers force thread friction and makes the seal without the use of a gasket.